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Flow Cytometry Facility |
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Department
of Oceanography, Texas A&M
University Tel: 979-862-7664 |
Our FACSCalibur utilizes a 15mW,
air-cooled argon with a fixed wavelength emission of 488nm. The FACSCalibur can
measure up to three fluorescent parameters (colors) as well as forward light
scatter (FSC) and side (or, right angle) light scatter (SSC). Our instrument is
also capable of sorting cell populations at a rate up to 300 cells/s. Cells can
either be sorted into 50ml tubes or directly onto filters for concentration and
subsequent analysis.
The
FACSCalibur has a closed fluidic system, so it is safe to use with biohazardous
samples if appropriate precautions are taken.
Typical
analysis (see
diagram) of marine picoplankton provides enumeration of:
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Prochlorococcus |
Synechococcus |
small eukaryotic algae |
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Cytograms
Analysis of flow
cytometry data to identify and enumerate Prochlorococcus (Pro), Synechococcus
(Syn), and small eukaryotic algae ("picoeukaryotes"or Peuk) based on
relative intensities of RFl and SSC parameters and the presence/absence of
ORFl.
A selection of other Flow Cytometry sites that provide useful information:
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Link |
To: |
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International Society
of Analytical Cytometry |
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The Scripps Research
Institute FACS Core Facility |
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University of
Massachusetts, Amherst Flow Cytometry Facility |
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University of
Texas Medical Branch, Galveston |
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Last modified: 23 June 2004